14389 Cav1 Status: ES cell colonies screened / QC no positives
| Gene | Cav1 |
| Chromosome | 6 |
| Deletion Start | 17,306,453 |
| Deletion End | 17,339,509 |
| Deletion Size | 33,057 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,307,052 | 17,307,071 | ACCACCGCCATTGTCAACTC |
| TUP | 17,307,074 | 17,307,099 | TCTTGGCACTCAGGTGGACTAAACGT |
| TUR | 17,307,128 | 17,307,150 | GGCTGTCTACTCCAAGCTGAATC |
| TDF | 17,339,311 | 17,339,331 | CCGTGCATCAAGAGCTTCCTG |
| TDP | 17,339,334 | 17,339,357 | TGAGATTCAGTGCATCAGCCGCGT |
| TDR | 17,339,367 | 17,339,389 | GATCGCAGAAGGTATGGACGTAG |
PCR Primers
| Primer | Sequence |
| SU | AACTGCCTAGCTCTGATGAAG |
| SD | AAACAAATGGCCGTAACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
