14395 Hmox1 Status: Cancelled
| Gene | Hmox1 |
| Chromosome | 8 |
| Deletion Start | 75,093,729 |
| Deletion End | 75,100,016 |
| Deletion Size | 6,288 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,093,912 | 75,093,929 | TCCTCTGACACGCTGGAG |
| TUP | 75,093,931 | 75,093,959 | TGACGATTGGGAGTGTCTTGTAGTATCCA |
| TUR | 75,093,961 | 75,093,980 | GCGTTCACCATCCCTCAAAC |
| TDF | 75,099,737 | 75,099,756 | GAGCAGTGGCTAGTGGTTTG |
| TDP | 75,099,762 | 75,099,785 | TAGCAGATCCTGGCCTTGGACTGG |
| TDR | 75,099,788 | 75,099,808 | TCGGTGTCATGTCTGCTACAG |
PCR Primers
| Primer | Sequence |
| SU | GCCAGAGTCATATGATTTATCCC |
| SD | GTCGTGGTCAGTCAACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
