| Gene | Snx18 |
| Chromosome | 13 |
| Deletion Start | 113,618,392 |
| Deletion End | 113,594,508 |
| Deletion Size | 23,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,617,761 | 113,617,780 | CAAGCGTCTGGAGCCAAGAG |
| TUP | 113,617,731 | 113,617,754 | CACGGTGAGCCGCAACCTCAATCG |
| TUR | 113,617,709 | 113,617,730 | CGACTTGACGAAGGTGGAGAAG |
| TDF | 113,595,428 | 113,595,449 | GCTGTCAGGCACATCATGTAGG |
| TDP | 113,595,405 | 113,595,427 | CTCGGGCTCTGGGTAAACCTGGC |
| TDR | 113,595,384 | 113,595,402 | TGTCAGGGCACCCAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCAATGGATTAGCTCTGCTG |
| SD | ACCCAGGTGGGACAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14409A-A4 | VGB6 | C57BL/6NTac | |
| 14409A-C2 | VGB6 | C57BL/6NTac | |
| 14409A-G11 | VGB6 | C57BL/6NTac | |
| 14409A-F5 | VGB6 | C57BL/6NTac | |
| 14409A-H4 | VGB6 | C57BL/6NTac | |
| 14409A-D5 | VGB6 | C57BL/6NTac | |
