14413 Twist1 Status: ES cell colonies screened / QC no positives
| Gene | Twist1 |
| Chromosome | 12 |
| Deletion Start | 33,957,981 |
| Deletion End | 33,958,623 |
| Deletion Size | 643 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,958,155 | 33,958,172 | ATCGGAGGCGGCGACGAG |
| TUP | 33,958,178 | 33,958,196 | CAGCCCGGCCCAGGGCAAG |
| TUR | 33,958,202 | 33,958,220 | TCCGCCCGCAGATTTCTTG |
| TDF | 33,958,418 | 33,958,440 | GAGCAAGATTCAGACCCTCAAAC |
| TDP | 33,958,441 | 33,958,463 | TGGCGGCCAGGTACATCGACTTC |
| TDR | 33,958,547 | 33,958,567 | TCCATCCTCCAGACGGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGGTATAAGAGCCGCCAAGT |
| SD | CAACCTGGAAGGATTTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
