| Gene | Pus3 |
| Chromosome | 9 |
| Deletion Start | 35,564,820 |
| Deletion End | 35,566,945 |
| Deletion Size | 2,126 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,565,395 | 35,565,416 | CACATTCTCAATCGGGTGCTTC |
| TUP | 35,565,418 | 35,565,442 | TGCAGACATCCGAGTATTGGCCTGG |
| TUR | 35,565,480 | 35,565,500 | AGCGATAAGTCCGCTCAAGAC |
| TDF | 35,566,714 | 35,566,733 | CCCTCATGGATCGTCCCAAG |
| TDP | 35,566,734 | 35,566,755 | TGCCAAGGACTGGAATCCCGGA |
| TDR | 35,566,823 | 35,566,842 | TGTCAGCACAGTCCCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTTATGAAGTGGCCAGATAGACTG |
| SD | TTAGGTGGATGCTGGGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14419A-D3 | VGB6 | C57BL/6NTac | |
| 14419A-F4 | VGB6 | C57BL/6NTac | |
| 14419A-H2 | VGB6 | C57BL/6NTac | |
