14433 Lrrc4 Status: ES cell colonies screened / QC positives
| Gene | Lrrc4 |
| Chromosome | 6 |
| Deletion Start | 28,831,611 |
| Deletion End | 28,829,607 |
| Deletion Size | 2,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,831,240 | 28,831,258 | TGGCCTCGCCAGTCTCAAC |
| TUP | 28,831,212 | 28,831,237 | TCTGGAACTGTTCGACAACTGGCTGA |
| TUR | 28,831,164 | 28,831,182 | GCTCCCGCAGTTTGGACAG |
| TDF | 28,830,083 | 28,830,104 | CAGTACCCTCCACAGATACCAC |
| TDP | 28,830,059 | 28,830,082 | TGACAAGATGCAGACCAGCCTGGA |
| TDR | 28,830,014 | 28,830,034 | GCCACAAAGCAGCCAATGATG |
PCR Primers
| Primer | Sequence |
| SU | CTTCCGAGGTAGGTGACAGG |
| SD | TTGACAGATGCAGGCTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
