14443 Trim33 Status: Cancelled
| Gene | Trim33 |
| Chromosome | 3 |
| Deletion Start | 103,279,882 |
| Deletion End | 103,311,508 |
| Deletion Size | 31,627 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,280,741 | 103,280,763 | GCCTGGTCCGACAGTGTTGATAG |
| TUP | 103,280,771 | 103,280,799 | TTGGAATGGATTGCTTATTGGCCTGTTCG |
| TUR | 103,280,858 | 103,280,878 | TGTCACCCTAGTGCCTTCTTC |
| TDF | 103,311,378 | 103,311,401 | CAGAGTCTGTTGGAACATCTGGTC |
| TDP | 103,311,404 | 103,311,433 | CGCCCAGTGTTTTGTCCTGTACATAAACAA |
| TDR | 103,311,468 | 103,311,489 | TGACAGTCTCTACACGTCAATC |
PCR Primers
| Primer | Sequence |
| SU | AGCTCCTACCGAGCTACACA |
| SD | TATCATACATAGCCACTAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
