14448 BC028528 Status: ES cell clone microinjected
| Gene | BC028528 |
| Chromosome | 3 |
| Deletion Start | 95,695,926 |
| Deletion End | 95,688,899 |
| Deletion Size | 7,028 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,695,537 | 95,695,557 | TCCTTCGGCAAGACTGAACTC |
| TUP | 95,695,505 | 95,695,530 | CTGTTCCTGTCACCACAGAGCTGAGC |
| TUR | 95,695,466 | 95,695,489 | AAGCGCCCACTTCTTTCCTTTATG |
| TDF | 95,689,636 | 95,689,654 | GTAAGCTGGGTGAGGTGTC |
| TDP | 95,689,608 | 95,689,634 | ACGACTGCAAGACAGAGACAGTTGACC |
| TDR | 95,689,587 | 95,689,606 | CCAGGCTGGCCTCTAATCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGGGAGGAAGCATAGGAGG |
| SD | TCTCTATGCAAACGCCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14448A-G1 | VGB6 | C57BL/6NTac | |
| 14448A-G3 | VGB6 | C57BL/6NTac | |
| 14448A-F2 | VGB6 | C57BL/6NTac | |
