14456 Grhl2 Status: ES cell colonies screened / QC no positives
| Gene | Grhl2 |
| Chromosome | 15 |
| Deletion Start | 37,233,390 |
| Deletion End | 37,270,781 |
| Deletion Size | 37,392 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,233,687 | 37,233,705 | GCTTCCTCCCCGACGAATG |
| TUP | 37,233,709 | 37,233,728 | TCAGCTCCCACTGCCCCGAC |
| TUR | 37,233,746 | 37,233,768 | CCATCTGCCAAAACCTCAATCAG |
| TDF | 37,270,413 | 37,270,435 | ACACAGTGAAGGTGTGAGTTGAG |
| TDP | 37,270,442 | 37,270,468 | AAAGTGCAGTGTGGTGGTCATTTCAGC |
| TDR | 37,270,489 | 37,270,513 | CGTGGTTACCCTGATTTGTATTTGC |
PCR Primers
| Primer | Sequence |
| SU | GCGAGAAAGTTACCTGTGGC |
| SD | ACAACTTCTCTGGACATGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
