14480 Vdr Status: Cancelled
| Gene | Vdr |
| Chromosome | 15 |
| Deletion Start | 97,884,936 |
| Deletion End | 97,857,270 |
| Deletion Size | 27,667 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,884,300 | 97,884,322 | AGAGCCTTCAATGGTTACCAAAC |
| TUP | 97,884,276 | 97,884,299 | CCGGTCTGTGCTGGACTCTTCACC |
| TUR | 97,884,189 | 97,884,208 | GGCCCTGACTGACCTAACAG |
| TDF | 97,857,435 | 97,857,457 | AGTACCGTTCCCTCTCCTTCCAG |
| TDP | 97,857,409 | 97,857,434 | CCGGAGAACAGCATGAAGCTCACACC |
| TDR | 97,857,380 | 97,857,398 | TCTCATTGCCGAACACCTC |
PCR Primers
| Primer | Sequence |
| SU | TTAGGTTTCCACACCCTTGC |
| SD | GGGATGATGGGTAGGTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
