14495 Rnase12 Status: ES cell colonies screened / QC no positives
| Gene | Rnase12 |
| Chromosome | 14 |
| Deletion Start | 51,057,217 |
| Deletion End | 51,056,733 |
| Deletion Size | 485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,057,170 | 51,057,192 | TGCTTCTGTTCTGGGAGAATGAG |
| TUP | 51,057,142 | 51,057,168 | TCACTGAAGATGTTGTGCTGACGTCCA |
| TUR | 51,057,048 | 51,057,065 | TGGTCGGGTTCCCTGATG |
| TDF | 51,056,819 | 51,056,843 | GTTTGTTCTTGTGACTTGTGATGAC |
| TDP | 51,056,790 | 51,056,816 | AGGGCCAGTTGATCTCCAGGGATATGT |
| TDR | 51,056,757 | 51,056,777 | TCCTCACACTCAGTGCTGATG |
PCR Primers
| Primer | Sequence |
| SU | CAGGGAGAGGAAGTGGAAGG |
| SD | GTTTGTGTGGTGCTTGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
