14498 Acadsb Status: ES cell colonies screened / QC no positives
| Gene | Acadsb |
| Chromosome | 7 |
| Deletion Start | 131,410,749 |
| Deletion End | 131,444,403 |
| Deletion Size | 33,655 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,411,643 | 131,411,664 | GCTTTCCTGGAGGATGTTTCTC |
| TUP | 131,411,669 | 131,411,693 | TAGGCGATTGACGAGCCCATTTAGC |
| TUR | 131,411,704 | 131,411,723 | GCACCCAACTGTCCTTTCAC |
| TDF | 131,444,041 | 131,444,060 | TCGTCTGTTCAAAGCTGGTG |
| TDP | 131,444,063 | 131,444,088 | AGTGGGCTGCAGATCAAATGGGAAAC |
| TDR | 131,444,126 | 131,444,147 | CCCTGCACTTGCTCTCAGTAAC |
PCR Primers
| Primer | Sequence |
| SU | GTGCCCTGAGTAACCGTAGC |
| SD | TTGTCAATTATATGGCCTACAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
