| Gene | Phka1 |
| Chromosome | X |
| Deletion Start | 102,643,921 |
| Deletion End | 102,615,916 |
| Deletion Size | 28,006 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,643,388 | 102,643,408 | CTCAGGCTGGCCTTGAACTTG |
| TUP | 102,643,356 | 102,643,382 | TTCCTGCCATAGCCTTCACAGTTTGGA |
| TUR | 102,643,336 | 102,643,355 | CACTGGCTTGTGGTCTGTTG |
| TDF | 102,616,023 | 102,616,040 | ACTGCCCAGGGCTTCAAC |
| TDP | 102,615,992 | 102,616,020 | CCAAAGAAGTTGATGCCAGTCTGCTCTCA |
| TDR | 102,615,946 | 102,615,967 | CCACCAAATGGCTGTCCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | GACACATTCTGGGCGGAG |
| SD | AATTGTAACGACTGCAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14514B-C5 | VGB6 | C57BL/6NTac | |
| 14514B-D5 | VGB6 | C57BL/6NTac | |
| 14514C-A5 | VGB6 | C57BL/6NTac | |
| 14514B-E9 | VGB6 | C57BL/6NTac | |
