14521   Rbmxl2     Status: ES cell colonies screened / QC no positives

GeneRbmxl2
Chromosome7
Deletion Start107,209,513
Deletion End107,210,718
Deletion Size1,206
Genome Build38
MGI IDMGI:1923822
NCBI Gene ID76572
BAC ID337D20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,209,542107,209,560CATCGGCGGTCTCAACCTC
TUP107,209,562107,209,583AGACCGACGAGAAGGGCCTCGA
TUR107,209,596107,209,616TCGATGATGCGGCCATACTTG
TDF107,210,275107,210,292GGCTACTGTGAGCGCGAC
TDP107,210,301107,210,318CGGCCGCGAGCGCGACTA
TDR107,210,327107,210,345CGGTAGGAGCCTCCGCTAG

PCR Primers

PrimerSequence
SUTAGGTTTGGAATGCTTTGGG
SDGAGTAGCAAACACCAATTCCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR71 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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