| Gene | C130074G19Rik |
| Chromosome | 1 |
| Deletion Start | 184,882,988 |
| Deletion End | 184,874,215 |
| Deletion Size | 8,774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 184,882,062 | 184,882,085 | CTCGGAATTATTGTCCAGGTAGTC |
| TUP | 184,882,034 | 184,882,059 | CAGTGGGTGACCTTCATCATACCCGA |
| TUR | 184,882,016 | 184,882,033 | GGCAAGGGACCGAATGTC |
| TDF | 184,874,388 | 184,874,408 | GCAAAGGATGCCGGTACATTG |
| TDP | 184,874,362 | 184,874,385 | ATTGGCCTGCAAGGATTTGCCGCA |
| TDR | 184,874,301 | 184,874,320 | GCTCTGGTTAGCGCACAAAG |
PCR Primers
| Primer | Sequence |
| SU | CGCACCCGTAGTAGGTCTG |
| SD | GTCAAGGCTAGTGCCGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14529A-F12 | VGB6 | C57BL/6NTac | |
| 14529A-C11 | VGB6 | C57BL/6NTac | |
| 14529A-B11 | VGB6 | C57BL/6NTac | |
| 14529A-D9 | VGB6 | C57BL/6NTac | |
