14534 Rhag Status: ES cell colonies screened / QC no positives
| Gene | Rhag |
| Chromosome | 17 |
| Deletion Start | 40,811,179 |
| Deletion End | 40,839,805 |
| Deletion Size | 28,627 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,811,247 | 40,811,270 | TGAATATGAGACACCACAGAATGC |
| TUP | 40,811,272 | 40,811,296 | TCCCAGAAGAATGCTTCCCACCAGA |
| TUR | 40,811,300 | 40,811,320 | AGGTGTTACCCTGCTGGGAAG |
| TDF | 40,838,954 | 40,838,974 | TCAGCAGTGAAGGGAGCATAG |
| TDP | 40,838,979 | 40,839,005 | CTTGTAGAGGACCTGGGTTCGTTTCCA |
| TDR | 40,839,025 | 40,839,046 | CTGGGAAATGGAGGTGGTTGTG |
PCR Primers
| Primer | Sequence |
| SU | TACCACATTCTACATTGTATGG |
| SD | AACTGCAGTGGGTAGGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
