14543 Ndufa1 Status: ES cell colonies screened / QC no positives
| Gene | Ndufa1 |
| Chromosome | X |
| Deletion Start | 37,191,087 |
| Deletion End | 37,187,698 |
| Deletion Size | 3,390 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,190,527 | 37,190,545 | GGCTAGGAGGCAGCAGAAG |
| TUP | 37,190,505 | 37,190,526 | TGGGTGCCACGAATCCAGTCCA |
| TUR | 37,190,467 | 37,190,486 | CCAGCTTTCCTGGACTTGAC |
| TDF | 37,188,638 | 37,188,660 | GCCTTGAACTCAGAGCAAACTTC |
| TDP | 37,188,615 | 37,188,636 | TGCCCTTGCCTCTAGTAGCCGA |
| TDR | 37,188,582 | 37,188,606 | CCTAATCCCAGTGAGTATTCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCACAGAGCTGCCTTCATC |
| SD | GACTCTTTGAGAAGTCTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
