14549 Sbp Status: ES cell colonies screened / QC no positives
| Gene | Sbp |
| Chromosome | 17 |
| Deletion Start | 23,942,449 |
| Deletion End | 23,945,489 |
| Deletion Size | 3,041 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,943,096 | 23,943,116 | CCTCTGCTCTGCTCCAGAAAG |
| TUP | 23,943,119 | 23,943,143 | AGGCATTTCCTTGGGACAGGCCAGG |
| TUR | 23,943,194 | 23,943,212 | TGGGCAATTAGCGACACTC |
| TDF | 23,945,169 | 23,945,189 | GGCCACCTTTGGTGTTAGAAG |
| TDP | 23,945,198 | 23,945,225 | ACTTCAGTGACACTGGAGGTTCAGACAA |
| TDR | 23,945,271 | 23,945,292 | CCATTTGAAGCCAATCCCTCTG |
PCR Primers
| Primer | Sequence |
| SU | AAAGGGAGAATGGGTTGAGC |
| SD | AGGAGGCTGGAGAACAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
