14563 Mcee Status: ES cell colonies screened / QC no positives
| Gene | Mcee |
| Chromosome | 7 |
| Deletion Start | 64,392,801 |
| Deletion End | 64,412,014 |
| Deletion Size | 19,214 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,393,449 | 64,393,471 | GCTTAAAGGGTGTTAGGCGAATG |
| TUP | 64,393,473 | 64,393,501 | ACTGGAAGGCCTGGAGAGTTCTGTCTGCT |
| TUR | 64,393,517 | 64,393,539 | AAGAGCAGACTTGGAAAGATGAC |
| TDF | 64,411,644 | 64,411,663 | TGGACCTCAGGGCTGACTAC |
| TDP | 64,411,667 | 64,411,694 | CACTCTGGGCTATGCTTCATGACTGAGC |
| TDR | 64,411,753 | 64,411,773 | GGCTTGATCTTGTGCCATCTG |
PCR Primers
| Primer | Sequence |
| SU | GTGTCGCGTGATTGCTTTC |
| SD | AGGTTAAATGAAGTGCCTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
