14566 BC056474 Status: ES cell colonies screened / QC no positives
| Gene | BC056474 |
| Chromosome | 8 |
| Deletion Start | 85,081,017 |
| Deletion End | 85,082,018 |
| Deletion Size | 1,002 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,081,038 | 85,081,056 | CCACGAAGGCCCAACAAAG |
| TUP | 85,081,060 | 85,081,083 | TGAGGTGAGCAGTCCAGCAAGTCA |
| TUR | 85,081,093 | 85,081,111 | CACGGCCACAACCAAAGAC |
| TDF | 85,081,797 | 85,081,820 | TGCTGTCTACTGCTCCTTTATCAG |
| TDP | 85,081,822 | 85,081,842 | TTTGCCAACTCGCGGAGCTCG |
| TDR | 85,081,868 | 85,081,887 | TCAGCATCCTCACATGAAGC |
PCR Primers
| Primer | Sequence |
| SU | TTTCAGCGCGTTAATGACAG |
| SD | GTCTTCACTGGGCAGAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
