| Gene | Hc |
| Chromosome | 2 |
| Deletion Start | 35,061,380 |
| Deletion End | 35,029,407 |
| Deletion Size | 31,974 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,061,027 | 35,061,050 | CAGCTTCTTGGCATTCATCACTTC |
| TUP | 35,060,992 | 35,061,019 | TTAACACTCAGTTCTCGCTTTCTGGGTC |
| TUR | 35,060,966 | 35,060,983 | GCGTTACCAGGGCTTCAC |
| TDF | 35,030,375 | 35,030,393 | TCCATCTAGCTGGGCTCAC |
| TDP | 35,030,351 | 35,030,373 | TTCCTCACCAACGCAAACGCAGA |
| TDR | 35,030,319 | 35,030,343 | TGTGGAGTGTCTTACCACGATAATG |
PCR Primers
| Primer | Sequence |
| SU | AGAAAGCCAGAGCCAAAGTG |
| SD | TACAAACAGAAAAGATGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14573A-H2 | VGB6 | C57BL/6NTac | |
| 14573A-D3 | VGB6 | C57BL/6NTac | |
| 14573A-D6 | VGB6 | C57BL/6NTac | |
| 14573A-C12 | VGB6 | C57BL/6NTac | |
| 14573A-C10 | VGB6 | C57BL/6NTac | |
