14583 Clcn7 Status: ES cell colonies screened / QC no positives
| Gene | Clcn7 |
| Chromosome | 17 |
| Deletion Start | 25,133,480 |
| Deletion End | 25,160,589 |
| Deletion Size | 27,110 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,134,248 | 25,134,271 | CAGCCAGACATGATAAGCAACAAG |
| TUP | 25,134,273 | 25,134,295 | CAGAGGCCAGAGTGTGCTCAGCA |
| TUR | 25,134,303 | 25,134,324 | AGGCATTTGAAGGCACAAACTG |
| TDF | 25,160,059 | 25,160,080 | GCTTGGTTTGGGAGTTTGAATC |
| TDP | 25,160,082 | 25,160,105 | CTTCCCATGCTGGGTAAAGTGGGC |
| TDR | 25,160,148 | 25,160,166 | CCCGGAAGAGCTTGAACAC |
PCR Primers
| Primer | Sequence |
| SU | CTCTTGGAGGACTAGGCGTG |
| SD | TGCAGACACAGGGTGAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
