14584 4930595M18Rik Status: ES cell colonies screened / QC no positives
| Gene | 4930595M18Rik |
| Chromosome | X |
| Deletion Start | 81,457,999 |
| Deletion End | 81,425,895 |
| Deletion Size | 32,105 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,457,694 | 81,457,713 | ATGCTCAACCCGATTGTAGC |
| TUP | 81,457,668 | 81,457,693 | TCGGGAAGGCAATACCAGTACAAGGA |
| TUR | 81,457,633 | 81,457,654 | GTCTTTCGCATTCGCTCTGAAG |
| TDF | 81,426,074 | 81,426,097 | TTGTACTTTGCAGGACTTCTTGTG |
| TDP | 81,426,039 | 81,426,064 | CTGGAGAACAACCAGAGGCAAGTGGA |
| TDR | 81,425,961 | 81,425,985 | GGACGTGTTATCAATGTGTGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGACTTGTTTAAACTGGAAAGCAG |
| SD | TTATCATCTATCTACTCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
