| Gene | Elfn2 |
| Chromosome | 15 |
| Deletion Start | 78,674,342 |
| Deletion End | 78,671,855 |
| Deletion Size | 2,488 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,673,682 | 78,673,702 | GTGAGTCACCGAGGGAGTTTG |
| TUP | 78,673,661 | 78,673,680 | CGGTTACCCGCTGCTCGTGC |
| TUR | 78,673,633 | 78,673,654 | ATGGCATTGAGGCTGTGGTAAG |
| TDF | 78,672,454 | 78,672,472 | CCGCAGTGAGCCGTAAGAC |
| TDP | 78,672,427 | 78,672,450 | CAGCGTGTCGTCCAGTGGCTCCAT |
| TDR | 78,672,393 | 78,672,413 | TGGCACATCCAGACTGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CATCTCGCTTAGCATCCCTC |
| SD | ACAAGAGGCCTGTGGTTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14598A-B10 | VGB6 | C57BL/6NTac | |
| 14598A-G4 | VGB6 | C57BL/6NTac | |
| 14598A-F9 | VGB6 | C57BL/6NTac | |
| 14598A-A3 | VGB6 | C57BL/6NTac | |
| 14598A-H3 | VGB6 | C57BL/6NTac | |
