| Gene | Aldh4a1 |
| Chromosome | 4 |
| Deletion Start | 139,623,142 |
| Deletion End | 139,648,317 |
| Deletion Size | 25,176 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,623,805 | 139,623,823 | CGGTGGGTGCCATAATGAC |
| TUP | 139,623,828 | 139,623,851 | ATAACGTGTGATGCGGCTCTGCGT |
| TUR | 139,623,930 | 139,623,949 | GCCTTGCACTCAGTGACCTG |
| TDF | 139,647,879 | 139,647,898 | ACGTGGCTACTGTGAACCTC |
| TDP | 139,647,901 | 139,647,924 | CCTCTTATCTGCCCTCCGGCCGTG |
| TDR | 139,647,960 | 139,647,979 | TCAGGGCTTGCTGAGCATAG |
PCR Primers
| Primer | Sequence |
| SU | GGATCTGTCATTGGCTCCTG |
| SD | CACCAGAGCTGTCTCCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14599A-A7 | VGB6 | C57BL/6NTac | |
| 14599A-C12 | VGB6 | C57BL/6NTac | |
| 14599A-H6 | VGB6 | C57BL/6NTac | |
| 14599A-E7 | VGB6 | C57BL/6NTac | |
| 14599A-A8 | VGB6 | C57BL/6NTac | |
| 14599A-E10 | VGB6 | C57BL/6NTac | |
