14604 Sox3 Status: ES cell colonies screened / QC no positives
| Gene | Sox3 |
| Chromosome | X |
| Deletion Start | 60,893,202 |
| Deletion End | 60,892,017 |
| Deletion Size | 1,186 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,892,870 | 60,892,890 | GACTGGAAACTGCTGACCGAT |
| TUP | 60,892,847 | 60,892,868 | CGGAGAAGCGGCCGTTCATCGA |
| TUR | 60,892,804 | 60,892,824 | CGGGTACTCCTTCATGTGCAC |
| TDF | 60,892,278 | 60,892,296 | AAGTCGGAGCCCAGCTCTC |
| TDP | 60,892,252 | 60,892,272 | CCCGCCATCGCTTCGCACTCG |
| TDR | 60,892,216 | 60,892,233 | GATCATGTCGCGCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGCAGGTATATAAGGGAC |
| SD | ACATTGGCTTTAGCTGTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
