| Gene | Kcna6 |
| Chromosome | 6 |
| Deletion Start | 126,739,921 |
| Deletion End | 126,738,282 |
| Deletion Size | 1,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,739,355 | 126,739,378 | GTCATCCTCATCTCCATCGTCATC |
| TUP | 126,739,332 | 126,739,353 | TTTGCCTGGAGACCTTGCCTCA |
| TUR | 126,739,291 | 126,739,311 | CACTACCCTCGTTGCTTCCAC |
| TDF | 126,738,441 | 126,738,459 | TGAAGGCAACGGACAATGG |
| TDP | 126,738,408 | 126,738,434 | CAAACCTGACTTTGCCGAGGCTTCACG |
| TDR | 126,738,372 | 126,738,392 | CGATGTGGAGTCGGAAGGTAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGAGAAGCGCAGTAGGAC |
| SD | TCCATTCTCCAGATTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14606A-A6 | VGB6 | C57BL/6NTac | |
| 14606A-D7 | VGB6 | C57BL/6NTac | |
| 14606A-E3 | VGB6 | C57BL/6NTac | |
| 14606A-H5 | VGB6 | C57BL/6NTac | |
