| Gene | 4930404N11Rik |
| Chromosome | 10 |
| Deletion Start | 81,365,783 |
| Deletion End | 81,364,064 |
| Deletion Size | 1,720 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,365,122 | 81,365,142 | AGGGCCATTCTCGGCTACAAG |
| TUP | 81,365,094 | 81,365,119 | ATAGACTGCAGGTGGGAAAGTGCACA |
| TUR | 81,365,052 | 81,365,071 | CAAGGTGCTGGGTGAGAGTC |
| TDF | 81,364,850 | 81,364,869 | CGGCCTGACCAACTCAGAAC |
| TDP | 81,364,825 | 81,364,849 | CTGGTGATGCCTGGTACAAGCTGCC |
| TDR | 81,364,754 | 81,364,773 | CACGCTGGCGACTCTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AAACAGGAACCCAAGTGGAG |
| SD | CAACCGTGGGTCTCCATTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14607A-A1 | VGB6 | C57BL/6NTac | |
| 14607A-B3 | VGB6 | C57BL/6NTac | |
| 14607A-D9 | VGB6 | C57BL/6NTac | |
| 14607A-E8 | VGB6 | C57BL/6NTac | |
| 14607A-E11 | VGB6 | C57BL/6NTac | |
| 14607A-F12 | VGB6 | C57BL/6NTac | |
