14611 Glrb Status: ES cell colonies screened / QC one positive
Gene | Glrb |
Chromosome | 3 |
Deletion Start | 80,912,086 |
Deletion End | 80,878,014 |
Deletion Size | 34,073 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 80,911,862 | 80,911,881 | ACAGCACTGTTTGCATCCAC |
TUP | 80,911,835 | 80,911,860 | CATGCACCTACTGTGTCCTGTTTGCA |
TUR | 80,911,785 | 80,911,805 | CCAACAGGAAGCCACCATCAC |
TDF | 80,878,752 | 80,878,772 | GCAGTCACAGTCTGGGTTGTC |
TDP | 80,878,724 | 80,878,749 | ATGCCTAGCATGCGTTGGTTCACACC |
TDR | 80,878,689 | 80,878,708 | GCCTTTAGCCTCCTAAGCAC |
PCR Primers
Primer | Sequence |
SU | TGAAATACTCTCCAACTTAATGCC |
SD | TCCCAGATGGAATACAAACAG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 97 bp | + | + | - |
TDF | TDR | 84 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |