| Gene | Loxl1 |
| Chromosome | 9 |
| Deletion Start | 58,312,883 |
| Deletion End | 58,288,830 |
| Deletion Size | 24,054 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,312,317 | 58,312,339 | CGCCCTTCGTAAACCAGTATGAG |
| TUP | 58,312,293 | 58,312,315 | ACTACGATCCCGCGTCACGGACC |
| TUR | 58,312,268 | 58,312,289 | GGTAGTACACGTAGCCCTGTTC |
| TDF | 58,289,298 | 58,289,321 | AGGTCTTATGAGGGTGTCCCTGTG |
| TDP | 58,289,275 | 58,289,295 | CCTGACCACAGGCAGGCTGCT |
| TDR | 58,289,231 | 58,289,251 | CCTTTCTGGGAACGCTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTCCAGACACTTTCAGCC |
| SD | TCCAGCAGCTACCCTTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14613A-D9 | VGB6 | C57BL/6NTac | |
| 14613A-G7 | VGB6 | C57BL/6NTac | |
| 14613A-E9 | VGB6 | C57BL/6NTac | |
