| Gene | Itsn2 |
| Chromosome | 12 |
| Deletion Start | 4,613,278 |
| Deletion End | 4,639,789 |
| Deletion Size | 26,512 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,613,284 | 4,613,305 | CAGTTTCCCACAGCGATGAATG |
| TUP | 4,613,308 | 4,613,335 | ATGTAACTTTGTGCATGGTAGGATGGGC |
| TUR | 4,613,339 | 4,613,360 | CAAATAGGCTTCCCACCACAAC |
| TDF | 4,639,296 | 4,639,319 | GATCATTGTCAGTATGCAGGAAGG |
| TDP | 4,639,321 | 4,639,346 | ACCTGAGGAGCACATGCTGTTAGCTG |
| TDR | 4,639,351 | 4,639,370 | CACGGCCTATCGTGGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCAAGGGTTTCTTCATTC |
| SD | GTGTTGCTGACATTACTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14622A-C7 | VGB6 | C57BL/6NTac | |
| 14622A-G9 | VGB6 | C57BL/6NTac | |
| 14622A-H9 | VGB6 | C57BL/6NTac | |
