14626 4931407G18Rik Status: Cancelled
| Gene | 4931407G18Rik |
| Chromosome | 5 |
| Deletion Start | 87,979,495 |
| Deletion End | 87,980,669 |
| Deletion Size | 1,175 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,979,983 | 87,980,002 | AGAGGACAGTGCTGCTGATG |
| TUP | 87,980,003 | 87,980,027 | TGGAGGATGCCTCTGGATTTCCAGA |
| TUR | 87,980,047 | 87,980,066 | GAGGTGGCAGAGGAAGGAAC |
| TDF | 87,980,395 | 87,980,413 | GCAGAGTCCCATGCTGTTC |
| TDP | 87,980,414 | 87,980,438 | TGCTCACTGCTGTGGAATCGAGGTA |
| TDR | 87,980,461 | 87,980,482 | CCTCCATGACGTTGTCAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | AACCACAAAGTACCTAGCAGGC |
| SD | TGGCTTCCTTTACTGCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
