14652 Slc4a4 Status: ES cell colonies screened / QC no positives
| Gene | Slc4a4 |
| Chromosome | 5 |
| Deletion Start | 88,934,753 |
| Deletion End | 88,954,953 |
| Deletion Size | 20,201 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,935,644 | 88,935,666 | CAAATGGAACCCACAGATGAACG |
| TUP | 88,935,668 | 88,935,691 | TGCGATCAGCTCCTCCTGTGAGTG |
| TUR | 88,935,722 | 88,935,745 | GAAATGCTAACCACAAGGACCAAC |
| TDF | 88,954,776 | 88,954,798 | AGGTCACCACACGATCTACATTG |
| TDP | 88,954,802 | 88,954,824 | TCCATGTGCCCAAGAGCTACCGG |
| TDR | 88,954,839 | 88,954,856 | TGCCCAGCCTTCCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | CTCTACATCCAGCCTACAAG |
| SD | GACTGTTGATGAGTGCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
