14653 Ogdh Status: Cancelled
| Gene | Ogdh |
| Chromosome | 11 |
| Deletion Start | 6,296,981 |
| Deletion End | 6,317,074 |
| Deletion Size | 20,094 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,297,312 | 6,297,331 | GCACCATCCACCATGTCTAC |
| TUP | 6,297,332 | 6,297,354 | TGCGTCAGACTCAGTGTGGCCTC |
| TUR | 6,297,368 | 6,297,389 | GCTGGAAACAACTCAAGAGGAC |
| TDF | 6,316,226 | 6,316,249 | GGAGGCAAACCTCGTAATAAGATG |
| TDP | 6,316,253 | 6,316,277 | CCATATCCATCCCTTACCCGGCACA |
| TDR | 6,316,278 | 6,316,299 | GAAGGGAAACGAAAGCTCAGAC |
PCR Primers
| Primer | Sequence |
| SU | CGAACACTCAATGGTTTCTGG |
| SD | GAGTCAGAAGAGCTACTATCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
