14662 4833423E24Rik Status: ES cell colonies screened / QC one positive
| Gene | 4833423E24Rik |
| Chromosome | 2 |
| Deletion Start | 85,518,763 |
| Deletion End | 85,484,041 |
| Deletion Size | 34,723 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,518,489 | 85,518,513 | GCAAGGTTTACAATGTGACTGACTG |
| TUP | 85,518,463 | 85,518,486 | CTGGCAAGCATCCAGGTGGACGTC |
| TUR | 85,518,438 | 85,518,457 | TGCCCAGCATAGTGGTTGAG |
| TDF | 85,484,153 | 85,484,174 | TTCCCTCTGGATGAATGCTTAC |
| TDP | 85,484,116 | 85,484,143 | TGCAGAGCCTTGCATTTATTGGTGTCTC |
| TDR | 85,484,093 | 85,484,114 | ATGCACAGCCATCACCCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AACAGCCAGCAAGGAGAGAG |
| SD | CACTGATTTCCAATGGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
