14663 Syn2 Status: ES cell colonies screened / QC no positives
| Gene | Syn2 |
| Chromosome | 6 |
| Deletion Start | 115,135,084 |
| Deletion End | 115,135,456 |
| Deletion Size | 373 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,135,105 | 115,135,125 | CTGTCGGACAGCAGCTTCATC |
| TUP | 115,135,129 | 115,135,147 | AACCTGCCCAATGGCTACA |
| TUR | 115,135,149 | 115,135,166 | GGGCGCTGAAGGTCAGTC |
| TDF | 115,135,326 | 115,135,346 | CAGCTTCTTCAGCTCGCTGTC |
| TDP | 115,135,352 | 115,135,372 | CGGTGAAGCAGACGGCCGCCT |
| TDR | 115,135,422 | 115,135,439 | ACCACCAGCAGCACCTTG |
PCR Primers
| Primer | Sequence |
| SU | AGACTCTCGGCTTTCCTTCC |
| SD | AAGCTCCATTGGTGCTTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
