14694 1700080E11Rik Status: ES cell colonies screened / QC no positives
| Gene | 1700080E11Rik |
| Chromosome | 9 |
| Deletion Start | 105,144,925 |
| Deletion End | 105,143,433 |
| Deletion Size | 1,493 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,144,857 | 105,144,874 | TGGCGGCATCTTTGTCAC |
| TUP | 105,144,831 | 105,144,852 | TGCTGTATGCGCCCGATCCAGG |
| TUR | 105,144,784 | 105,144,805 | TCACCAGCACAGCATAGTTCAG |
| TDF | 105,143,542 | 105,143,560 | CGCCAGGGAACAGCTACTG |
| TDP | 105,143,513 | 105,143,537 | CCCTCCAGGACTTGGGAATTCTGGC |
| TDR | 105,143,433 | 105,143,452 | TCTCGGCTCAGGTCCCAATC |
PCR Primers
| Primer | Sequence |
| SU | CCTACATTCAGCTCCAACCC |
| SD | TCGAGTGACCACGAAGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
