14696 Atp2c1 Status: Cancelled
| Gene | Atp2c1 |
| Chromosome | 9 |
| Deletion Start | 105,494,417 |
| Deletion End | 105,464,632 |
| Deletion Size | 29,786 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,494,273 | 105,494,293 | GCCCTTTGCATGTGAAATGTC |
| TUP | 105,494,245 | 105,494,272 | TGAGGATTTGGTTCCCTAAGGGCATGTA |
| TUR | 105,494,176 | 105,494,197 | TTCCCAGGAAAGCAGACTCAAG |
| TDF | 105,464,746 | 105,464,768 | TTTAGTGCTGGGTTTCATTCTCC |
| TDP | 105,464,716 | 105,464,745 | AGGGTCTGCATAGCCAGAGAAACACAGTGT |
| TDR | 105,464,686 | 105,464,710 | TCAGACATTCGCAAGAACTAACTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCTCCTGGTTTCTCTTG |
| SD | TGGTGGATTATATTGCTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
