14719 Fancc Status: ES cell colonies screened / QC no positives
| Gene | Fancc |
| Chromosome | 13 |
| Deletion Start | 63,403,003 |
| Deletion End | 63,398,088 |
| Deletion Size | 4,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,402,974 | 63,402,992 | TGCAGACCTTGCTTCTGAC |
| TUP | 63,402,948 | 63,402,973 | TGTCAGTCTTGGCTGCAGAAGCTTTC |
| TUR | 63,402,886 | 63,402,908 | ACCCGGACAAGTGAAGACAAGTG |
| TDF | 63,398,700 | 63,398,722 | TCCTGTCCAGTGCCTTTCATAAC |
| TDP | 63,398,663 | 63,398,691 | TGGAAGCACTATTAGCTTACAGGAGGCTG |
| TDR | 63,398,637 | 63,398,659 | CTCAAAGACATCCAGAGTTCACC |
PCR Primers
| Primer | Sequence |
| SU | AATGGCTCAATCGTCTGGAG |
| SD | TCATCATAAGGAGCAGTACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
