Gene | Sdhaf1 |
Chromosome | 7 |
Deletion Start | 30,322,326 |
Deletion End | 30,321,936 |
Deletion Size | 391 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 30,322,296 | 30,322,314 | CGGTTGCAGAGGCAAGTTC |
TUP | 30,322,271 | 30,322,291 | CCTGTACCGCGAGCTGCTGCG |
TUR | 30,322,169 | 30,322,191 | TACAGATACTCGATACGCAGCAC |
TDF | 30,322,038 | 30,322,056 | GGGACCAGGCTGGATGATG |
TDP | 30,322,019 | 30,322,036 | TGGTGCCCCAAAGAATTC |
TDR | 30,321,949 | 30,321,970 | GGAACCTTCCTCTAGGTCTGTC |
PCR Primers
Primer | Sequence |
SU | ATGCTTCCACTCAAGTTCAGA |
SD | TAATCTCCCTCATGAACCCG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 146 bp | + | + | - |
TDF | TDR | 108 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
14720A-C11 | VGB6 | C57BL/6NTac | |
14720A-C12 | VGB6 | C57BL/6NTac | |
14720A-F6 | VGB6 | C57BL/6NTac | |
14720A-D3 | VGB6 | C57BL/6NTac | |