14740   Prmt6     Status: ES cell colonies screened / QC no positives

GenePrmt6
Chromosome3
Deletion Start110,250,968
Deletion End110,249,803
Deletion Size1,166
Genome Build38
MGI IDMGI:2139971
NCBI Gene ID99890
BAC ID221L3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF110,250,771110,250,790AGGAGATGATCGCCGACCAG
TUP110,250,746110,250,768CCGCACCGAAGCCTACCGCTTAG
TUR110,250,696110,250,714CACGTCCAGCACCGTCTTG
TDF110,250,339110,250,356TGGCGTCTGGGTTTCTGG
TDP110,250,313110,250,336CCAGGTGAAGCAGCACTATGGCGT
TDR110,250,247110,250,266TGCACCACGATCTCCGAATG

PCR Primers

PrimerSequence
SUGAAAGGGCAAAAACTGAAAGG
SDAGACCATGTTGCCTTTCTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR95 bp++-
TDFTDR110 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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