| Gene | Foxg1 |
| Chromosome | 12 |
| Deletion Start | 49,384,489 |
| Deletion End | 49,385,938 |
| Deletion Size | 1,450 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,384,968 | 49,384,989 | CAAGGAGGGCGACAAGAAGAAC |
| TUP | 49,384,992 | 49,385,017 | CAAGTACGAGAAGCCGCCGTTCAGCT |
| TUR | 49,385,040 | 49,385,058 | CTCGGGACTCTGCCTGATG |
| TDF | 49,385,422 | 49,385,442 | GCCAGCAGCACTTTGAGTTAC |
| TDP | 49,385,443 | 49,385,464 | AACGGGACCACGTCGGCCTACC |
| TDR | 49,385,538 | 49,385,555 | TCCACACTCAGCCCGTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTGCTGCTGCTGTGACTG |
| SD | CTGCACACATGGAAATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 134 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14751A-F6 | VGB6 | C57BL/6NTac | |
| 14751A-C6 | VGB6 | C57BL/6NTac | |
| 14751A-H6 | VGB6 | C57BL/6NTac | |
