| Gene | Apbb2 |
| Chromosome | 5 |
| Deletion Start | 66,462,113 |
| Deletion End | 66,451,474 |
| Deletion Size | 10,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,461,273 | 66,461,292 | GCTGAGGTAAGGGCTCTGTG |
| TUP | 66,461,242 | 66,461,270 | TCAGCCTATAGATTGTCACCCGAGATGGG |
| TUR | 66,461,221 | 66,461,240 | TGCAACCCTGAGAAGTCCTG |
| TDF | 66,452,249 | 66,452,269 | GGCACCTTGGCAGTGTTTATG |
| TDP | 66,452,223 | 66,452,247 | CCAGCAGCGGATCCACAGACATTGC |
| TDR | 66,452,169 | 66,452,188 | TGGGAGGAACGGAGATTGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGAGACAGGCAGACATGC |
| SD | AGTCAAATGTCAGAGAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14762A-B9 | VGB6 | C57BL/6NTac | |
| 14762A-E10 | VGB6 | C57BL/6NTac | |
