14765 Atr Status: ES cell colonies screened / QC no positives
| Gene | Atr |
| Chromosome | 9 |
| Deletion Start | 95,857,728 |
| Deletion End | 95,883,203 |
| Deletion Size | 25,476 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,858,243 | 95,858,261 | CGCGTTGGTGTTCATCTGC |
| TUP | 95,858,267 | 95,858,287 | CCTCTTTGAGGCTCGCCGCCT |
| TUR | 95,858,289 | 95,858,309 | GCCACCCAAATTCTGGGATTG |
| TDF | 95,882,847 | 95,882,869 | AGTAAGGCTCTTCCTCATCTGTC |
| TDP | 95,882,871 | 95,882,900 | TCAGCCATGTTGTAACCCTAAGGTAACCTA |
| TDR | 95,882,904 | 95,882,922 | CTTGACGCTGAGGCACCTG |
PCR Primers
| Primer | Sequence |
| SU | AACTTTGTTGGGTCGTCAGG |
| SD | TCCTAACTCACATCATCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
