| Gene | Ifitm3 |
| Chromosome | 7 |
| Deletion Start | 141,010,642 |
| Deletion End | 141,009,705 |
| Deletion Size | 938 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,010,329 | 141,010,349 | GAACCTGAAGCTCCATCTACC |
| TUP | 141,010,303 | 141,010,328 | CAGATGGCACCGAGGTATGAGTGTGG |
| TUR | 141,010,267 | 141,010,298 | GAAGAATGAACACAAACTTATTTTAAACAAAG |
| TDF | 141,009,957 | 141,009,975 | TCCACAGGGAGTACGTCAG |
| TDP | 141,009,924 | 141,009,952 | ACTGTCAGAGCATCTGCCTATCAGCTCCA |
| TDR | 141,009,887 | 141,009,909 | AGACTGGAGCAGAACAGATAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGAGGTTCTGCAGCTGTG |
| SD | CGTCCAGAAGAATACGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14769A-D7 | VGB6 | C57BL/6NTac | |
| 14769A-F11 | VGB6 | C57BL/6NTac | |
| 14769A-H9 | VGB6 | C57BL/6NTac | |
| 14769A-B8 | VGB6 | C57BL/6NTac | |
| 14769A-H11 | VGB6 | C57BL/6NTac | |
