14772 Ano5 Status: ES cell colonies screened / QC no positives
| Gene | Ano5 |
| Chromosome | 7 |
| Deletion Start | 51,511,238 |
| Deletion End | 51,537,830 |
| Deletion Size | 26,593 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,511,961 | 51,511,985 | TGTCTTGGCATGTTCTAAACTTCAG |
| TUP | 51,511,987 | 51,512,016 | CATGTGCAGCACCATTCCTTGTATTTGACA |
| TUR | 51,512,021 | 51,512,041 | GCTTCCTCCACAAGGGAATAC |
| TDF | 51,537,727 | 51,537,748 | GAGAAGTGAGCACAGCAAAGAC |
| TDP | 51,537,753 | 51,537,779 | TCTTCTTCAGAGATGGAATCCGGCAGA |
| TDR | 51,537,784 | 51,537,804 | TCCACGTAGGACAGGACAAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAGGAAATCCAGTCAGCG |
| SD | CAAGGTATGAAGTAGATATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
