14773 Hfe2 Status: ES cell colonies screened / QC no positives
| Gene | Hfe2 |
| Chromosome | 3 |
| Deletion Start | 96,526,518 |
| Deletion End | 96,528,707 |
| Deletion Size | 2,190 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,526,562 | 96,526,581 | TCTAAGCACCCTCACTCTCC |
| TUP | 96,526,582 | 96,526,604 | TGCTGCTCCTCTGTGGACAGGGT |
| TUR | 96,526,640 | 96,526,659 | TCCTACCCAGCATCCTAACC |
| TDF | 96,528,451 | 96,528,472 | GAAGGGCTTCCGGTTGAAGATG |
| TDP | 96,528,476 | 96,528,504 | ACTTCCAATCCTGCGTCTTTGATGTTTCA |
| TDR | 96,528,519 | 96,528,539 | TGAGCTGCCACAGTAAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCATATTGCACAAGGGAC |
| SD | GACAATGCCCACTTCGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
