14773 Hfe2 Status: ES cell colonies screened / QC no positives
Gene | Hfe2 |
Chromosome | 3 |
Deletion Start | 96,526,518 |
Deletion End | 96,528,707 |
Deletion Size | 2,190 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 96,526,562 | 96,526,581 | TCTAAGCACCCTCACTCTCC |
TUP | 96,526,582 | 96,526,604 | TGCTGCTCCTCTGTGGACAGGGT |
TUR | 96,526,640 | 96,526,659 | TCCTACCCAGCATCCTAACC |
TDF | 96,528,451 | 96,528,472 | GAAGGGCTTCCGGTTGAAGATG |
TDP | 96,528,476 | 96,528,504 | ACTTCCAATCCTGCGTCTTTGATGTTTCA |
TDR | 96,528,519 | 96,528,539 | TGAGCTGCCACAGTAAAGTTG |
PCR Primers
Primer | Sequence |
SU | AGGCATATTGCACAAGGGAC |
SD | GACAATGCCCACTTCGTTTC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 98 bp | + | + | - |
TDF | TDR | 89 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |