14778   Zic2     Status: ES cell colonies screened / QC no positives

GeneZic2
Chromosome14
Deletion Start122,475,679
Deletion End122,479,305
Deletion Size3,627
Genome Build38
MGI IDMGI:106679
NCBI Gene ID22772
BAC ID279G17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 14

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,475,811122,475,830CAGAACGGCTTCGTGGACTC
TUP122,475,839122,475,860CGCACATGGGCGCCTTCAAGCT
TUR122,475,894122,475,911GACGTGAACGCCGAACTC
TDF122,479,187122,479,206GGGACAACCGGAGGCCATAG
TDP122,479,207122,479,234CGGCCTCTCCTCCAACTTCAATGAATGG
TDR122,479,262122,479,284GGTGGATAAGGGAACAGGGAATG

PCR Primers

PrimerSequence
SUCTCCTTTAATACACTTTGGTTC
SDGGAAGGGAAATGGGAGAAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR101 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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