14782 Usf1 Status: ES cell colonies screened / QC no positives
| Gene | Usf1 |
| Chromosome | 1 |
| Deletion Start | 171,414,525 |
| Deletion End | 171,418,193 |
| Deletion Size | 3,669 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGGCTGGCTTCAAACTCAC |
| TUP | | | ATCCACCTGCCTGTCTCCTGAGTGC |
| TUR | | | GTATTACTGCTGGCTCATGGTG |
| TDF | | | CCACCCTGGACTTGGACTATTATC |
| TDP | | | AGGAAGTCAGAGGCTCCCAGGACAAC |
| TDR | | | GAGCCCTCCGTTTCTCATC |
PCR Primers
| Primer | Sequence |
| SU | CCTGAGTGTTAGGCTCCCAG |
| SD | CAGCCAGCTAGCATCCTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
