14802 Ddx6 Status: ES cell colonies screened / QC no positives
| Gene | Ddx6 |
| Chromosome | 9 |
| Deletion Start | 44,607,396 |
| Deletion End | 44,635,895 |
| Deletion Size | 28,500 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,607,887 | 44,607,909 | GCAGGCTTACTTGGTAGCCAAAG |
| TUP | 44,607,913 | 44,607,939 | ACCTGGAACCTGTGGCAATCTTCCTGT |
| TUR | 44,607,956 | 44,607,977 | TGGCTCACAGGTGTAACCTTAG |
| TDF | 44,635,792 | 44,635,814 | CAACATCGACAAGAGCCTGTATG |
| TDP | 44,635,815 | 44,635,839 | TGGCAGAATACCACAGTGAGCCTGC |
| TDR | 44,635,847 | 44,635,867 | ACGTGCTTGTTACGGTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGCCTGAGTGTGGCTGTTAG |
| SD | AGGCAGCCTTCATTCACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
